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VANTAstar – Microplate reader

Flexible microplate reader with simplified workflow
The new VANTAstarTM is the latest addition. This compact multimode microplate reader is conceived for ease of use and flexibility, and ensures easy detection setup for simple data generation. Equipped with patented dual LVF Monochromators™ systems, filters, UV/vis spectrometers and Advanced Dynamic Range technology, it can cover a wide range of applications in basic research and science learn life.

  • LVF Monochromators for wavelength flexibility and performance
  • Automatic sensitivity adjustment with EDR
  • Fast autofocus on the entire plate
  • Optimized luminescence detection with automatic crosstalk reduction
  • Scanning at absorbance, fluorescence and luminescence
  • Dedicated features for live cell-based assays
  • Comprehensive capabilities with top/bottom probes and auto switches
  • Compact, space-saving stand
  • Easy integration into automated systems
Detection mode Cuong +B1:B69uang (including FRET)
Fluorescence polarization
Luminescent (flashes and glows) – BRET included
Time-resolved fluorescence
TR-FRET
UV/vis . absorption spectrum
Measurement mode Read above and below
Endpoints and kinetics
Sequential Multiple Stimulation
Sequential multicast
Scan spectrum (fluorescence, luminescence, absorbance)
Scale measurements
Sweep the well
Microplate form 6-384 well plate, user definable
LVis plate with 16 low-volume microspots (2 μL)
Microplate holder Compatible with robots
Light source High-energy xenon flash.
Probe Low noise photomultiplier tube
CCD . Spectrophotometer
Wavelength selection Dual Linear Variable Filter (LVF) Monochromators™
Linear transform dichroic mirror
Separate monochromator LVF excitation and emission
Optical filter:
Excitation and emission sliders hold 5 filters on each side
LVF Monochromators + optical filter
Use one for excitation and the other for emission
UV/Vis Absorption Spectrometer: Full spectrum or 8 separate wavelengths for <1 second/well
Spectral band Filter
FI, LUM, TRF, FP, TR-FRET: 240-740 nm.
LVF Monochromators™
FI, LUM: 320 – 740 nm.
Dichroic Linear Variation
340 – 740 nm.
Spectrum
ABS: 220 – 1000 nm; wavelength accuracy: 0.5 nm
Sensitivity FI filter (top)
<0.5 pM (<10 amol/FITC well, 384sv, 20 μL)
FI filter (bottom)
<2.5 pM (<125 amol/FITC well, 384g, 50 μL)
Monochrome FI (top)
<0.8 pM (<16 amol/FITC well, 384sv, 20 μL)
Monochrome FI (bottom)
<3.5 pM (<175 amol/FITC well, 384g, 50 μL)
Dynamic range: 8 decades in a single measurement
FP
<1.2 mP SD at 1 nM FITC (384 s, 20 μL)
HTRF® (black and white microplate)
Reader controller (Eu) after 18h (384sv, 20 μL)
Delta F > 700% (High Corrector)
Delta F > 25% (Low Corrector)
TRF
<30 fM europium (<2.4 amol/well, 384, 80 μL)
LUM
<0.8 pM (<15 amol/ATP well, 384sv, 20 μL)
Dynamic range: 8 decades in a single measurement
ABS with spectrometer
Selectable spectral resolutions: 1, 2, 5 and 10 nm
OD range: 0 to 4 OD; photometric resolution: 0.001 OD
Accuracy: <1% at 2 OD
Accuracy: <0.5% at 1 OD and <0.8% at 2 OD
Linearity: 0.8% at 2.0 OD
Reading time Flight mode (flashes once)
9 seconds (96), 16 seconds (384)
Shake Linear, wobble and double wobble with user-defined time and speed
INCUBATE +4°C above ambient up to 45°C or 60°C
Minimal condensation concept: the upper heating plate of the annealing chamber operates at +0.5°C above the lower plate, the optical system is heated
Software Includes MARS Data Analysis Software and Intelligent Control for multiple users
FDA 21 CFR Part 11 Compliant
Built-in fluorophore library
Size Width: 35cm, depth: 52cm, height: 36cm; Weight: 27 kg
Optical filter Excitation and emission sliders up to 4 filters for monochromator (VANTAstar) and up to 5 filters per filter only (VANTAstar F).
Optical path guide Top and bottom: free-air optical light path guided by motor-driven and dichroic mirror
Z-Adjustment Auto focus height adjustment (0.1 mm resolution)
Injection reagents Up to 2 reagent injectors
Individual injection volumes per well: 3 to 500 μL (up to 1 mL optional)
Variable spray speed up to 420 μL/s
Reagents are flushed
Heater and stirring plate
Size: width: 13cm, depth: 31cm, height: 26cm; Weight: 6 kg

 

Plate Lvis Sample capacity: 16 separate microdrop wells for 2 µL of sample; one standard cuvette position for up to 1 mL sample
Internal standard quality control (optional): 04 NIST traceable optical density filters (approximate values 0.1, 0.3, 0.6 and 1.0 OD); a holmium oxide filter for wavelength accuracy.
Size: conforms to SBS standards for microsamples.
THERMOSSTAR Microplate incubators and shakers
Air Controller (ACU) Actively regulate O2 and CO2 from 1-20%
Optical filter Excitation and emission filters
Upgrade Upgrades are available to include options like additional detection modes, reagent nozzles, extended temperature control, etc.

 

  • Quantification of DNA, RNA and proteins
  • ELISA and DELFIA immunoassays
  • Bacterial Growth (OD600)
  • Cell viability/toxicity
  • Real-time cell-based tests
  • Enzyme activity
  • Reporter gene testing…