Filter-based multimode microplate reader
FLUOstar® Omega is a multimode microplate reader with 6 detection modes. It uses an ultrafast UV/vis spectrophotometer or filters for absorbance as well as high sensitivity filters for all other detection modes. FLUOstar Omega is the ideal plate reader for life science applications.
| Detection mode | Fluorescence intensity – including FRET |
| AlphaScreen ®/AlphaLISA® | |
| Luminescent (flashes and glows) – including FRET | |
| Time-resolved fluorescence | |
| TR-FRET | |
| Absorption spectrum UV/Vis | |
| Measurement mode | Read above and below |
| Endpoints and kinetics | |
| Sequential Multiple Stimulation | |
| Multi-sequential emission | |
| Scan spectrum (absorbance) | |
| Scale measurements | |
| Microplate form | Plates 6 to 384, user definable |
| LVis plate with 16 low-volume microspots (2 μL) | |
| Microplate holder | Compatible with robots |
| Light source | High-energy xenon flash |
| Probe | Low noise photomultiplier tube |
| Spectrophotometer CCD | |
| Wavelength selection | Optical filters: excitation and emission rotary heads for 8 filters per side |
| UV/vis absorption spectrometer: full spectrum or 8 separate wavelengths for <1 second/well | |
| Spectral band | Filter |
| 240-740 nm or 240-900 nm for FI, FP, TRF | |
| 240 – 740 nm for LUM | |
| Spectrometer | |
| 220 – 1000 nm for ABS; wavelength accuracy: 0.5 nm | |
| Sensitivity | FI |
| <0.2 fmol/well FITC | |
| TRF | |
| <30 amol/well europium | |
| Premium TRF for Omega | |
| Premium TRF for Omega | |
| LUM | |
| <20 amol/well ATP | |
| AlphaScreen® | |
| 5 pM (<100 amol/well P-Tyr-100, 384sv, 20 μL) | |
| ABS with spectrometer | |
| Selectable spectral resolutions: 1, 2, 5 and 10 nm | |
| OD range: 0 to 4 OD; photometric resolution: 0.001 OD | |
| Accuracy: <1% at 2 OD | |
| Accuracy: <0.5% at 1 OD and <0.8% at 2 OD | |
| Linearity: 0.8% at 2.0 OD | |
| Reading time | Flight mode (1 flash) |
| 9 seconds (96), 16 seconds (384) | |
| Shake | Linear, orbital and dual with user-defined time and speed |
| Clean the vent hole | System for pumping air or vacuum into the reader |
| Annealing mode | +4°C above ambient up to 45°C or 65°C |
| The upper heating plate of the annealing chamber operates at a temperature 0.5°C higher than the lower plate. This prevents steam build-up on the lid or sealing gasket. | |
| Software | Includes multi-user reader control software and MARS data analysis software |
| Follow FDA 21 CFR Part 11 | |
| Size | Width: 44cm, depth: 48cm, height: 30cm; Weight: 28 kg |
| Optical filter | Excitation and emission rotary head for 8 filters per side |
| Optical path guide | Above: liquid filled light guides |
| Bottom: optical fiber | |
| Injection reagents | Up to 2 reagent injectors built in |
| Inject at measuring site (6 to 384 wells) | |
| Individual injection volumes per well (3 to 500L) | |
| Variable injection speed up to 420 L/s | |
| Up to four injections per well | |
| Reagents are flushed |
| LVis Plate | Sample capacity: 16 separate microdrop wells for 2 µL of sample; one standard cuvette position for up to 1 mL sample |
| Internal standard quality control (optional): 04 NIST traceable optical density filters (approximate values 0.1, 0.3, 0.6 and 1.0 OD); a holmium oxide filter for wavelength accuracy | |
| Size: conforms to SBS standards for microsamples | |
| THERMOstar | Microroplate incubators and shakers |
| Air Controller (ACU) | Actively regulate O2 and CO2 from 0.1-20% |
| Stacker | Can hold up to 50 plates, providing continuous loading capacity |
| Optical filter | Excitation and emission filters |
| Upgrade |
Please contact your local representative for an upgrade that includes options such as detection modes, reagent injectors, etc. |
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UV/vis SPECTROstar Omega – Absorption plate reader with spectrometer
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