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FLUOstar Omega – Microplate reader

Filter-based multimode microplate reader
FLUOstar® Omega is a multimode microplate reader with 6 detection modes. It uses an ultrafast UV/vis spectrophotometer or filters for absorbance as well as high sensitivity filters for all other detection modes. FLUOstar Omega is the ideal plate reader for life science applications.

  • High speed absorbance measurements
  • Plate measurements up to 384 wells
  • Read below and above
  • Annealing mode up to 45 or 65°C
  • Many shaking options
  • Well scan with up to 900 data points per well
  • Nozzle high precision with simultaneous injection and reagent detection
  • Low volume measurements
  • Easy integration into automation systems



Detection mode Fluorescence intensity – including FRET
AlphaScreen ®/AlphaLISA®
Luminescent (flashes and glows) – including FRET
Time-resolved fluorescence
Absorption spectrum UV/Vis
Measurement mode Read above and below
Endpoints and kinetics
Sequential Multiple Stimulation
Multi-sequential emission
Scan spectrum (absorbance)
Scale measurements
Microplate form Plates 6 to 384, user definable
LVis plate with 16 low-volume microspots (2 μL)
Microplate holder Compatible with robots
Light source High-energy xenon flash
Probe Low noise photomultiplier tube
Spectrophotometer CCD
Wavelength selection Optical filters: excitation and emission rotary heads for 8 filters per side
UV/vis absorption spectrometer: full spectrum or 8 separate wavelengths for <1 second/well
Spectral band Filter
240-740 nm or 240-900 nm for FI, FP, TRF
240 – 740 nm for LUM
220 – 1000 nm for ABS; wavelength accuracy: 0.5 nm
Sensitivity FI
<0.2 fmol/well FITC
<30 amol/well europium
Premium TRF for Omega
Premium TRF for Omega
<20 amol/well ATP
5 pM (<100 amol/well P-Tyr-100, 384sv, 20 μL)
ABS with spectrometer
Selectable spectral resolutions: 1, 2, 5 and 10 nm
OD range: 0 to 4 OD; photometric resolution: 0.001 OD
Accuracy: <1% at 2 OD
Accuracy: <0.5% at 1 OD and <0.8% at 2 OD
Linearity: 0.8% at 2.0 OD
Reading time Flight mode (1 flash)
9 seconds (96), 16 seconds (384)
Shake Linear, orbital and dual with user-defined time and speed
Clean the vent hole System for pumping air or vacuum into the reader
Annealing mode +4°C above ambient up to 45°C or 65°C
The upper heating plate of the annealing chamber operates at a temperature 0.5°C higher than the lower plate. This prevents steam build-up on the lid or sealing gasket.
Software Includes multi-user reader control software and MARS data analysis software
Follow FDA 21 CFR Part 11
Size Width: 44cm, depth: 48cm, height: 30cm; Weight: 28 kg
Optical filter Excitation and emission rotary head for 8 filters per side
Optical path guide Above: liquid filled light guides
Bottom: optical fiber
Injection reagents Up to 2 reagent injectors built in
Inject at measuring site (6 to 384 wells)
Individual injection volumes per well (3 to 500L)
Variable injection speed up to 420 L/s
Up to four injections per well
Reagents are flushed
LVis Plate   Sample capacity: 16 separate microdrop wells for 2 µL of sample; one standard cuvette position for up to 1 mL sample
Internal standard quality control (optional): 04 NIST traceable optical density filters (approximate values 0.1, 0.3, 0.6 and 1.0 OD); a holmium oxide filter for wavelength accuracy
Size: conforms to SBS standards for microsamples
THERMOstar Microroplate incubators and shakers
Air Controller (ACU) Actively regulate O2 and CO2  from 0.1-20%
Stacker Can hold up to 50 plates, providing continuous loading capacity
Optical filter Excitation and emission filters
Please contact your local representative for an upgrade that includes options such as detection modes, reagent injectors, etc.
  • 260/280 ratio measurement for DNA and RNA, purity assessment
  • Biomolecular interaction tests
  • Cell-based assays
  • Link test
  • Enzyme activity test
  • Quantitative tests
  • Monitor bacterial growth