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CLARIOstar Plus – Microplate reader

The most versatile plate reader for test development
CLARIOstar® Plus is the most versatile multi-mode plate reader. It is equipped with patented LVF Monochromators™, filters and spectrometers. Flexibility combined with best-in-class sensitivity and newly developed Advanced Dynamic Range technology, make it the ideal reader for test development.

  • Innovative monochromators for increased sensitivity
  • Totally flexible wavelengths and bandwidths
  • Scan the spectrum at absorbance, fluorescence and luminescence
  • Dynamic range maximum  without manual intervention
  • Fast autofocus across the entire plate
  • Dedicated features for live cell-based assays
  • Extremely fast data sampling (100 measurements/second)
  • Specialized Lasers for Alpha Technology
  • Easy integration into automated systems
Detection mode Fluorescence intensity (including FRET)
Fluorescence polarization
AlphaScreen® / AlphaLISA® / AlphaPlex™
Luminescent (flashes and glows) – BRET included
Time-resolved fluorescence
UV/vis . absorption spectrum
Measurement mode Read above and below
Endpoints and kinetics
Sequential Multiple Stimulation
Sequential multicast
Scan spectrum (fluorescence, luminescence, absorbance)
Scale measurements
Sweep the well
Microplate form 6 – 1536 wells, user definable
LVis plate with 16 low-volume microspots (2 μL).
Microplate holder Compatible with robots
Light source High-energy xenon flash
Specialized lasers for AlphaScreen® / AlphaLISA® / AlphaPlex™.
Probe The photomultiplier tube has low noise.
Red-shifted photomultiplier tube
CCD Spectrophotometer
Wavelength selection Monochromators™ Dual Linear Transform Filters (LVFs).
Linear transform dichroic mirror.
Separate mono set LVF ex & em.
Optical filter:
ex & em sliders hold 4 filters on each side
LVF Monochromators + optical filter
Use one for ex and the other for em
UV/Vis Absorption Spectrophotometer:
Full spectrum or 8 separate wavelengths in <1 second/well
Spectral band Filter
FI, FP, TRF: 240-740 nm or 240-900 nm (red-shifted PMT)
LUM: 240 – 740 nm
LVF Monochromators™
FI: 320 – 740 nm or 320 – 840 nm (red-shifted PMT)
LUM: 320 – 740 nm
Linear Variation Dichroism
340 – 740 nm or 340 – 760 nm (red-shifted PMT)
ABS: 220 – 1000 nm; wavelength accuracy: 0.5 nm
Sensitivity FI filter (top)
<0.15 pM (<3 amol/FITC well, 384sv, 20 μL)
FI filter (bottom)
<1.0 pM (<50 amol/FITC well, 384g, 50 μL)
Monochrome FI (top)
<0.35 pM (<7 amol/FITC well, 384sv, 20 μL)
Monochrome FI (bottom)
<3.0 pM (<150 amol/FITC well, 384g, 50 μL)
Dynamic range: 8 decades in a single measurement
<0.5 mP SD at 1 nM FITC (384 s, 20 μL)
HTRF® (black and white microplate)
Reader controller (Eu) after 18h (384sv, 20 μL)
Delta F > 880% (High Corrector)
Delta F > 30% (Low Corrector)
<20 fM europium, 384, 80 μL
<0.4 pM (<8 amol/ATP well, 384sv, 20 μL)
Dynamic range: 8 decades in a single measurement
AlphaScreen® with laser
<5 pM (<100 amol/well P-Tyr-100, 384sv, 20 μL)
ABS with spectrometer
Selectable spectral resolutions: 1, 2, 5 and 10 nm
OD range: 0 to 4 OD; photometric resolution: 0.001 OD
Accuracy: <1% at 2 OD
Accuracy: <0.5% at 1 OD and <0.8% at 2 OD
Linearity: 0.8% at 2.0 OD
Reading time Flight mode (flashes once)
8 seconds (96), 15 seconds (384), 28 seconds (1536)
10 flashes
19 seconds (96), 57 seconds (384), 3 minutes 4 seconds (1536)
Shake Linear, orbital and dual trajectories with user-defined time and speed.
INCUBATE +3°C above ambient up to 45°C or 65°C
The upper heating plate of the annealing chamber operates at a temperature 0.5°C higher than the lower plate. This prevents steam build-up on the lid or sealing gasket.
Software Includes multi-user reader control software and MARS data analysis software.
FDA 21 CFR Part 11 Compliant
Integrated fluorophore library


Plate Lvis Sample capacity: 16 separate microdrop wells for 2 µL of sample; one standard cuvette position for up to 1 mL sample
Internal standard quality control (optional): 4 NIST traceable optical density filters (approximate values 0.1, 0.3, 0.6 and 1.0 OD); a holmium oxide filter for wavelength accuracy
Size: conforms to SBS standards for microsamples
THERMOSSTAR Microplate incubators and shakers
Air Controller (ACU) Actively regulate O2 and CO2 from 0.1-20%.
Stacker Can hold up to 50 plates, providing continuous loading capacity
Optical filter Excitation and emission filters
Upgrade CLARIOstar Plus may include all or any combination of the features listed below upon purchase. Upgradeable with additional features at any time.


  • Quantification of DNA, RNA and proteins
  • Fluorescence and luminescence assay development
  • Real-time and cell-based tests
  • Protein-protein interactions
  • Molecular Bonding Test
  • Enzyme activity
  • Reporter gene test