Hydra Bio – Focused Ion Beam Scanning Electron Microscope (FIB-SEM)

The unique Thermo Scientific Spin Mill Bio Method on the Hydra Bio Plasma-FIB provides large-area planar milling up to 1 mm in diameter, with a geometry like microtome-based serial block face imaging but at a slice thickness as small as 5 nm. Uncover and visualize large sample areas with Spin Mill Bio Method, then focus in on your regions of interest. It is also ideal to prepare clean and smooth surfaces. The Spin Mill Bio Method is fully automated and easy to set up using guided software. Multiple areas for image acquisition within one experiment can be selected. Each area of interest can be imaged at different imaging settings based on the specificity of the experiment.  Sparse features can easily be identified, and statistically relevant 3D data can be collected from multiple areas.

The Easylift Cryo Lift-Out System supports “on the grid” and bulk high pressure frozen (HPF) specimen techniques to help confirm region of interest in final lamella.  Fluorescently labeled regions of interested are carefully localized using the iFLM Correlative System, and then extracted and thinned before transferring for tomographic data collection in a cryo-TEM.

The integrated iFLM Correlative System combines fluorescence and electron imaging with ion milling and guided lamella production and supports correlative (CLEM) workflows. Identify, target, and confirm regions of interest without the hassle of transferring between instruments.