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rapifleX ® MALDI PharmaPulse ®

The rapifleX® MALDI PharmaPulse® accelerates the drug discovery process utilizing label-free mass spectrometry for uHTS.

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rapifleX® MALDI PharmaPulse®

The all new rapifleX® MALDI Time-of-Flight mass spectrometer is the heart of the MALDI PharmaPulse®, it incorporates Brukers latest 10 kHz scanning laser technology and can read multiple samples per second. All components of the system work in concert for fully automatic HTS operation. The newly designed screening software suite supports HTS workflows and works in combination with a proprietary assay development module which empowers assay developer to accelerate label-free assay development for MALDI mass spectrometry. The latest ‚Synthesis Screening‘ software module of the rapifleX® MPP opens a new application space for e.g. monitoring of chemical reactions at high pace or analysis of binding assays at incomparable high throughput and specificity.

rapifleX® MALDI PharmaPulse® (MPP) combs through large compound libraries of millions of substances

rapifleX MALDI PharmaPulse
Automated Sample Preparation for uHTS – with Courtesy of Analytik Jena

The use of mass spectrometry allows the measurement of unmodified substrates in primary screens to avoid false positives or false negatives, making confirmation screens redundant. The system is designed for fully automatic handling of 384 and 1536 sample plates to screen more than a million compounds in a week in support of drug discovery.

New standard for ultra-High-Throughput Screening Label-Free ‘Hit’ Identification

By combining the speed of Bruker’s patented smartbeam 3D laser technology with the direct-detection capability of mass spectrometry, the rapifleX® MPP delivers unprecedented screening capability for ultra high-throughput screening of biochemical and cellular assays. It allows you to screen through large and diverse compound libraries at a pace of over a million compounds a week, epitomizing the perfect balance of automation and miniaturization.

Robustness to analyze 2-3 million samples without intervention

rapifleX MALDI PharmaPulse
CyBio Well vario for uHTS– with Courtesy of Analytik Jena
  • New source and ion optical design
  • No source/ion optic cleaning even within primary screens
  • Easy and fast cleaning if required

Compatible with CyBio®-Well vario with 384 or 1536 channel simultaneous pipettor for high precision and ultra-fast sample plate preparation.

Single digit cost per sample

  • No LC solvents, no waste, no labeling
  • 0.025 to 1 μL sample volume
  • Low cost disposable sample plates

Fully automatic 1536 sample plate change

  • New ultra-fast autoloader seamlessly integrable with common robot systems
  • Rugged design tailored for uHTS operation

New disposable MALDI HTS sample plates

  • Fulfills all requirements for reliable MALDI ionization
  • Suitable for 384, 1536, 3072 or 6144 geometries

 

Speed
Less than a second per sample.
Robustness
Maximum uptime in 24/7 operation and full automation.
Confidence
Label-free mass spectrometry for lowest false discovery rates.
Flexibility
Enhancing performance for a wide range of screening applications.

The rapifleX® MPP assists assay development, accelerates HTS and uHTS hit finding, and supports hit confirmation and lead optimization

The MPP system allows for the measurement of unmodified substrates in primary biochemical screens, and offers several advantages:

  • It effectively eliminates confirmatory assays typically required of traditional fluorescence-based screening techniques
  • Saves costs for developing specific fluorescence probes or antibodies
  • Doesn’t require expensive solvents or consumables for continuous operation
  • Allows sample volume down to 25 nL

PharmaPulse Solution

The PharmaPulse Solution is based on Bruker´s advanced MALDI-TOF technology and on a dedicated software package. Ease of use and time to result were key drivers during the development resulting in an intuitive but yet powerful software solution to measure and analyze millions of samples in full automation. Two workflows guide the user from adapting an assay to MALDI mass spectrometry to readily exported results for further analysis in LIMS systems.

New software module supports synthesis screening and binding assays

rapifleX MALDI PharmaPulse Lead Generation Support
rapifleX® MPP in support of lead optimization

In biochemical screening experiments, the rapifleX® MPP determines the rate of conversion in a biochemical assay when performed in the presence of potential inhibitors. In such a screen, a distinct MS peak ratio comparing product and substrate intensities is monitored throughout large numbers of sample spots, where each spot represents the same biochemical conversion reaction, but measures the effectiveness of a different inhibitor molecule.

In addition to this screening functionality, the new MPP software module supports “Synthesis Screening”, a methodology that requires more flexibility with regard to m/z values to be targeted by MALDI-TOF MS. Here, m/z values of interest may vary from spot to spot as each sample may represent a different chemical reaction. Accordingly, the new MPP “Synthesis Screening” software module allows scientists to screen for a large number of compounds per individual sample spot. This new MALDI-TOF based high-throughput analysis workflow could, therefore, enable highly time-efficient surveying of chemical reactivity landscapes at previously unseen mapping depth.

rapifleX® MPP – the universal tool for drug discovery

rapifleX MALDI PharmaPulse

Full library screens

The rapifleX® MPP is the only mass spectrometer for large primary screens of millions of compounds with high diversity. Full automation supports screening of more than one million compounds per week, avoiding time-consuming confirmatory screens.

rapifleX MALDI PharmaPulse
MALDI PharmaPulse® assay development module to support assay development for HTS

Focused library screens

The quality of data obtained with rapifleX® MPP meets the requirements of secondary and confirmatory screens and makes it the ideal detector for focused library screens, such as the identification of kinase inhibitors of a target protein from a smaller subset of the library.

Fragment screening

The mass range and resolution accessible by MALDI-TOF mass spectrometry enables screening of fragments and other low molecular weight compounds.

Target evaluation and hit confirmation

Thorough understanding of enzyme biochemistry and kinetics is essential to develop and validate robust HTS assays. rapiflex® MPP assists assay development and hit confirmation with linearity tests, substrate selection, Ki measurements and IC50 determination.

IC50 determination of inhibitors

Half-maximal inhibitor concentration (IC50) is a key parameter in the drug discovery process. MALDI PharmaPulse® software supports assay development by featuring a dedicated method development module. Optimal assay conditions obtained using the method development module are applied during MALDI-TOF screening, for example aiming for IC50 determination, which is set up and executed in the software´s dedicated screening module.

rapifleX® MPP – for primary screening

Quy trình khám phá thuốc với rapifleX MALDI PharmaPulse®

A million samples per week with rapifleX® MALDI PharmaPulse®

The new rapifleX® MPP is the result of Bruker’s innovative development of MALDI mass spectrometers. It reflects our passion for and knowledge about this technology and its unique applications. The time-of-flight mass spectrometer (TOF MS) of the rapifleX® MALDI PharmaPulse® monitors up to 30 masses in HTS mode: from small molecules to large proteins. The improved resolution and sensitivity of the rapifleX® TOF MS allows e.g. the monitoring of acetylcholine (ACh) and choline to identify acetylcholinesterase (AChE) inhibitors out of millions of substances. An ingenious detector design delivers significantly improved signal to noise ratios for enhanced sensitivity across the mass range. A completely new HTS software suite supports easy set-up of HTS screens, and the proprietary assay development module ensures fast adaption of label-free assays for MALDI mass spectrometry.

Automation of rapifleX® MALDI PharmaPulse®

The rapifleX® MALDI PharmaPulse® is designed to seamlessly integrate with laboratory automation systems to perform fully automatic screening campaigns. The MALDI PharmaPulse® software solution offers an open interface to control parts of its operation from external scheduling software (e.g. Analytik Jena lab automation, HighRes Biosolutions, Beckman Coulter, Thermo Scientific automation). The new high-speed autoloader of the rapifleX® MPP works hand in hand with robot systems for quick loading and unloading of sample plates.

Mối tương quan giữa Khối phổ MALDI với Khối phổ SPE
Correlation between MALDI mass spectrometry (1536 plate geometry) with SPE mass spectrometry (384 plate geometry). Haslam, C. et al., The Evolution of MALDI-TOF Mass Spectrometry toward Ultra-High-Throughput Screening: 1536-Well Format and Beyond. J. Biomol. Screen. 2015, 1-11.

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