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DS5 – Dual Beam UV-Vis Spectrophotometer

The DS5 UV-Vis Spectrophotometer is a high performance dual beam instrument that measures absorption and transmission as a function of wavelength and is suitable for many analytical applications where accuracy and precision measurements are key to your results.

Designed and manufactured in the UK, the DS5 provides a modern, user-friendly and accurate UV-Vis spectrophotometer for a wide range of sample types and measurements.

Utilising a dual lamp and Czerny-Turner configuration monochromator, the DS5 features a compact, reliable and high throughput optical system which ensures impressive spectral performance. Additional benefits include stray light, baseline flatness, wavelength and photometric accuracy and reproducibility.

  • Variable bandpass options at 0.5, 1.0, 1.5, 2.0 or 4.0 nm over
    the range 190 nm – 1100 nm
  • Fast scanning – up to 6,000 nm/min to aid sample analysis throughput
  • Automatic accessory recognition connected to the DS5
  • Optional Visacle® software for PC control
  • Modern touchscreen interface with intuitive menus and functions to ensure ease of use for standalone control
  • USB, SD card and internal data storage for convenient retrieval of methods and results

Photometry Mode: Perform quantitative analyses in either absorbance or transmittance modes. Select from single up to 5 different individual wavelengths, nucleic acid/protein A260/A280 ratios and set up calibration curves for concentration measurements.

Time Scan: Perform kinetic measurements for time periods ranging from 10 seconds to >27 hours. Measurement intervals are factory preset and automatically selected when the scan time is set.

Wavelength scan: Perform a full spectral scan from 190 nm – 1100 nm at any of 9 incremental and preset selectable scan speeds starting from a high resolution 10 nm/min up to a maximum scan speed of 6,000 nm/min. Data is displayed as a graphical spectrum on which data analysis can be performed through the touchscreen interface with zoom, peak-valley, smoothing and other functions available through the easy to use menu interface.

  • Concentration measurement
  • Absorbance/Transmittance measurement
  • Hexavalent Chromium, Nucleic acid measurement (nucleic acid purity, nucleic acid concentration, protein concentration calculation)
  • Wavelength scan
  • Time change
  • Single and multi-wavelength photometry

Validation Functions

To ensure optimum instrument performance, self-diagnosis incorporating a number of parameters and wavelength calibration are automatically initiated upon start-up. Furthermore, the DS5 is equipped with a GLP/GMP feature for analyses requiring validation and auditing. Parameters such as wavelength accuracy, wavelength reproducibility, bandpass, baseline flatness, baseline stability and noise level can all be validated.

Data Handling

  • Scale changing, Trace, Spectrum overlay, Peak & trough detection, Smoothing, Differentiation, and Area & rate calculating
  • Internal memory, External USB, SD card
  • File output in CSV and text format

Optional Visacle® PC-controlled Software

Visacle® offers all control, validation, data analysis and reporting functionality for DS5 in one PC-operated software package. Collected data is stored in a secure database, where it can then be organised and analysed, as well as exported in .txt, .csv or PDF format.

Visacle® can be purchased as a CFR Part 11 compliant package for users requiring additional security. Features include:

  • Configurable user profiles with secure login and e-signatures
  • Audit trail logging of all actions taken
  • Permission system to restrict actions and data access to authorised users