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Vitamin D plays a vital role in skeletal metabolism, calcium homeostasis, and also in the functioning of the immune, cardiovascular, and reproductive systems. Vitamin D deficiency leads to rickets and osteomalacia and is also associated with breast and colorectal cancers, multiple sclerosis, dementia, rheumatoid arthritis, diabetes, Parkinson’s and Alzheimer’s diseases.

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Consequently, laboratory testing of serum vitamin D levels has experienced phenomenal growth over the past several years. LC-MS/MS is currently the best technique available for the correct quantification of 25-Hydroxy Vitamin D3 and 25-Hydroxy Vitamin D2.Jasem method provides analysis of 25-Hydroxyvitamin D3, 25-Hydroxyvitamin D2 and 3-epi-25-Hydroxyvitamin D3 in serum samples using simple sample preparation technique without SPE, on-line SPE or evaporation.

  • No need SPE, on-line SPE or evaporation
  • Use of only one standard HPLC system and one column
  • Quick and easy sample preparation
  • Sufficient chromatographic separation of 3-epi-25-hydroxy vitamin D3 in 8.5 minutes with accurate quantification