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rapifleX® MALDI Tissuetyper®

The rapifleX® MALDI Tissuetyper® re-defines the key performance measures for MALDI Imaging

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Laser and ion source designed for MALDI tissue typing

During the image acquisition the sample stage moves continually to achieve high pixel rates. The unique smartbeam 3D laser fires at a repetition rate of up to 10 kHz.

The smartbeam 3D laser features a laser diameter of 5 µm, enabling MALDI Imaging of tissue at 20 µm spatial resolution. As illustrated in the video, the laser can move independently from the sample stage to scan the full area of each pixel. This produces truly square pixels which utilizes the total available sample area for maximum sensitivity and pixel-to-pixel reproducibility.

Integrated software solutions

  • flexImaging dedicated molecular histology software controls data acquisition, data visualization, annotation and integration of virtual microscopic slides
  • SCiLS™ Lab is the dedicated software for statistical analysis. From individual imaging datasets to complete clinical biomarker studies, it features univariate and multivariate methods to mine the data and to find relevant information
Speed
Up to 40 true pixels / second for faster and better images.
Definition
smartbeam 3D laser for better sensitivity, True Pixel MALDI Imaging and pixel-to-pixel reproducibility.
Robustness
Novel ion source design for increased robustness and maximized uptime.
Ease of use
With the new ease-of-use workflow, MALDI Imaging becomes a de-skilled routine application. Employing IntelliSlides® supporting automation features and fleXmatrix® for higher throughput.

Novel ion source design for robust day-to-day operation

MALDI Imaging data overlaid with virtual microscopic slide in flexImaging
MALDI Imaging data overlaid with virtual microscopic slide in flexImaging
  • Extends uptime between cleaning by orders of magnitude
  • Easy access to self-aligning ion lenses when cleaning is in fact needed. No tools are needed to extract the whole first lens package which then can be rinsed with Ethanol, dried and re-inserted

Leading Software for MALDI tissue typing

flexImaging allows to set up and analyze individual datasets

  • Further improved automation
  • Wizard driven user-friendly set up of new experiments
  • True 64-bit application allows efficient handling of megapixel datasets
  • Direct access to single mass spectra
  • Batch acquisition
  • Co-register and super-impose virtual microscopic slides at full resolution for detailed histological annotation

 

 

 

SCiLS™ Lab

3D volume reconstruction and spatial segmentation of a kidney sample in SCiLS Lab.
Distribution of three distinct m/z values in a 3D reconstruction of 49 sagittal mouse brain sections containing a non-metastasizing cerebellar tumor with SCiLS™ Lab.

Integrating with SCiLS™ Lab Pro for statistical analysis allows automatic assignment of tissue classes.

With the unparalleled speed of the rapifleX®, 3D MALDI Tissuetyper® studies are within reach. The SCiLS™ Lab Premium 3D option allows the reconstruction, visualization, and statistical analysis of 3D Imaging datasets.

Tissue typing – A clinical discovery workflow

Tissue typing - A Clinical Discovery Workflow

The speed of the rapifleX® Tissuetyper® allows for fast acquisition of images and SCiLS™ Lab solution seamlessly allows for classification of the data sets, enabling tissue typing at minimal time. Tissue micro arrays can be analyzed very rapidly. The soft smartbeam 3D laser effectively ionizes the analytes but does not harm the tissue, in fact histological staining is possible after the acquisition of the MALDI data set.

The combination of the mass spectrometric, molecular information with the histology of the analyzed tissue yields information-rich data sets, resulting in deeper understanding of the spatial distribution of molecules linked to the observed biology. The image displays a typical workflow, and also shows some statistical features of the SCiLS™ Lab solution

 

 

 

 

 

 

 

 

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