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SpectraMax® Paradigm – Multi-Mode Microplate Reader

High-throughput screening capability on one fast, configurable microplate reader

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Z-height and microplate optimization increases assay dynamic range and sensitivity

The SpectraMax® Paradigm® Multi-Mode Microplate Reader measures absorbance, fluorescence, time-resolved fluorescence (including HTRF), fluorescence polarization, AlphaScreen®, AlphaLISA®, and luminescence assays for up to 1536-well plates. Detection cartridge modularity allows for easy addition of new read modes within minutes without requiring a service visit, while providing the flexibility for future applications.

Get automatic Z-height optimization Get automatic Z-height optimization

Z-height focusing is enabled for fast top- and bottom-reads for all read modes. By performing Z-height optimization, a lens is adjusted to address low volumes or varied well geometry.

Eliminate manual gain adjustment Eliminate manual gain adjustment

The detection cartridge utilizes an energy intensity optimization technique allowing you to read up to seven logs of sample concentration in a single pass of a plate without manual gain adjustment.

Tailor your data analysis Tailor your data analysis

Perform multiple calculations on one data set, compare multiple experiments side-by-side, and adjust reduction parameters while collecting real-time data with SoftMax® Pro Software.

ValitaTiter plates

  • 4x Faster than existing methods: Fast, homogeneous, and automation-friendly assay with results for 96 samples in less than 15 minutes
  • Expedite time to market: Reliably monitor protein titer in real-time throughout the bioprocess to rapidly adjust the process conditions for maximum protein output while minimizing process time.
  • Reduce development risk: Measure and monitor human IgG production at multiple time points to ensure quality and reduce development risk.
Read modes Absorbance

Fluorescence (top/bottom read)

Luminescence (top/bottom read)

Time-Resolved Fluorescence (top/bottom read)

Fluorescence Polarization (top/bottom read)

HTRF (top/bottom read)

AlphaScreen (top/bottom read)
Wavelength ranges Abs: 230 – 1000 nm

FL Ex: 360 – 790 nm

FL Em: 400 – 850 nm

Lumi: 360 – 850 nm

TRF Ex: 360 – 790 nm

TRF Em: 400 – 850 nm
Wavelength selection Cartridge filters and monochromator tunable in 1 nm increments
Absorbance photometric accuracy/linearity Cartridge: ±(2% + 0.010) OD at 2 OD
Absorbance photometric precision/repeatability Cartridge: ±(1% + 0.005) OD at 2 OD
Sensitivity top read optimized FL cartridge: 0.1 fmol/well in 75 μL fluorescein 384 well, 0.03 fmol/well in 8 μL fluorescein 1536 well

Lumi cartridge: 0.3 fmol/well in 200 μL ATP-Glow 96 well (1.5 pM), 0.2 fmol/well in 50 μL ATP-Glow 384 well (4 pM) TRF cartridge: 3 amol/well in 100 μL europium (0.03 pM) 384 well, 1 amol/well in 8 μL europium (0.125 pM) 1535 well

FP cartridge: 3 mP 75 μl fluorescein 384 well, 6 mP 8μl fluorescein 1536 well

AlphaScreen cartridge: <100 amol/well 384 well
Light source(s) Xenon flash lamp Ultra high power LEDs
Detector(s) Silicon photodiode Photomultiplier tube
Read types (endpoint, kinetic, etc) Endpoint Kinetic Spectrum scan* Well scan*
Read times Abs: 19 seconds

FL: 13 seconds

Lumi: 11 seconds

TRF: 12 seconds

FP: 17 seconds AS: 22 seconds
Plate shaking Linear Orbital
Western Blot true
Robotics / automation true
Plate type(s) 6-1536 well plates
Temperature control Ambient + 4°C to 45°C
Dimensions H 45 cm x W 39.2 cm x D 63 cm
Validation tools Multi-mode validation plate SoftMax® Pro GxP Software
Absorbance, Fluorescence and Luminescence true
Time-Resolved Fluorescence (TRF) true
Fluorescence Polarization (FP) true
Htrf true
Alpha Screen true
Imaging
Couvette Port
Injectors

Configuration changes in minutes

Adding new read modes is fast and simple with the detection cartridge system. Order additional detection cartridges as your application needs change, and install them by yourself in under two minutes.

Increased dynamic range and sensitivity

Use predefined reductions, columns, and summary formulas, or create custom calculations. Analyze EC50 and Z-factors to identify assay quality, and graph results with over 21 curve fit options.

Absorbance

Common absorbance applications and assays including ELISAs, nucleic acid and protein quantitation, and microbial growth.

AlphaScreen

AlphaScreen® Technology is a homogeneous, high-throughput method for detecting intramolecular binding. Donor and acceptor beads are attached to biomolecules, and when the beads are brought into proximity (via binding), a cascade of chemical reactions occurs, amplifying a signal that is detected on the SpectraMax® i3x and Paradigm® Multi-Mode Microplate Readers.

ELISA

ELISA (Enzyme-linked immunosorbent assays)

Enzyme-linked immunosorbent assays (ELISAs) are used to measure the amount of a specific protein, using a microplate format, and results are most often detected via absorbance in the visible wavelength range. Chemiluminescent and fluorescent ELISA formats offer enhanced sensitivity for accurate quantitation of less abundant analytes.

Fluorescence

Many fluorescence-based assays include cell viability, GPCR activity, and fluorescent nucleic acid quantification.

Fluorescence Polarization (FP)

Fluorescence polarization (FP) is a technique that is widely used to monitor binding events in solution. It can be used to assess biomolecular interactions, including protein-antibody binding and DNA hybridization, as well as enzyme activity, and it has been adapted to basic research as well as high-throughput screening.

HTRF

HTRF®, an assay technology developed by Cisbio, combines standard FRET (fluorescence resonance energy transfer) with time-resolved measurement of fluorescence, eliminating short-lived background fluorescence. HTRF’s applications include assessment of small phosphorylated peptides, immunoassays for quantifying large glycoproteins, and indirect detection of tagged complexes such as CD28/CD86 via secondary antibodies. These homogeneous assays do not require wash steps and are amenable to small-volume microplate formats used in high-throughput screening.
(This assay is suitable for SpectraMax® iD5 and SpectraMax® Paradigm readers.)

IgG Quantification

Immunoglobulin G (IgG) Quantification

In therapeutic protein engineering and cell line development, IgG quantification is the process of measuring the amount of immunoglobulin G (IgG), a common class of therapeutic proteins, produced by a genetically modified cell line. This is important for evaluating and monitoring the productivity of the cell line and selecting the best candidate for further development of therapeutic antibodies.

Kinase/Phosphatase Assays

Kinases are one of the most critical targets in drug discovery today. These enzymes are key components in cellular signaling pathways, and disruption to their function causes a variety of diseases, such as metabolic diseases, certain cancers, and cardiac disease. The functionally related phosphatases and phosphodiesterases are also important screening targets.

Luminescence

The key luminescence-based assays include dual-luciferase reporter gene, chemiluminescent ELISA, cytotoxicity, and BRET.

TRF, TR-FRET & HTRF

Time-resolved fluorescence – TRF and TR-FRET including HTRF assays provide applications resources to aid in your research, including kinase assays, cellular signaling pathways, protein-protein interactions, cell cytotoxicity, and more.

Western blotting

Western blotting is a popular technique used for protein detection and quantitation. Molecular Devices introduce a unique Western blot detection system on a multi-mode microplate reader, including colorimetric, fluorescence, and chemiluminescence detection methods.

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