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SpectraMax® Mini – Multi-Mode Microplate Reader

Affordable Maxline multi-mode microplate reader with best-in-class data analysis software

Perform your favorite applications including ELISA, DNA and protein quantitation, cell viability, gene expression with a user-friendly reader

The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget friendly solution that gives you exactly what you need when you need it. With three modes of detection for UV-Vis absorbance, fluorescence and luminescence, the SpectraMax Mini reader is user upgradeable – from 2 to 3 modes, providing researchers with a versatile instrument that does not compromise on sensitivity or performance. Included SoftMax® Pro Software enables rapid data generation and analysis helping you achieve more in less time.

Easy-to-use Easy-to-use

Save time on training, data generation, and data analysis, for faster results.

Flexible Flexible

Perform your favorite applications with a user-friendly reader that accommodates plate types from six to 384-well formats; up to three modes of detection measuring absorbance with NFC-tagged filters for fluorescence, luminescence, or both.

Affordable Affordable

Gain access to over thirty years of experience and technology in a compact, multi-mode reader that was built for researchers with sensitive budgets.

Save time and money with a microplate reader and washer bundle

Microplate Reader and Washer Bundle

Improve your lab’s efficiency, get more data, and decrease time to discovery with an automated microplate washer and reader solution:

  • The SpectraMax® Mini Multi-Mode Microplate Reader offers a budget-friendly solution with three modes of detection for UV-Vis absorbance, fluorescence, and luminescence.
  • SoftMax Pro Software is an industry-leading acquisition and analysis software with over 160 ready-to-run protocols, powerful data analysis, and a range of data output options.
  • The MultiWash+™ Microplate Washer is an automated, compact, quiet, efficient washer with 20 different wash protocol options.

General specifications

Dimensions (in.) 15.75 (L) x 12.60 (W) x 13.78 (H)
Dimensions (cm) 40 (L) x 32 (W) x 35 (H)
Power requirement External 24V DC power supply compatible with 100–240V AC @50–60 Hz 160W maximum consumption
Weight ≤ 40 lbs (18 kg)
Robotic compatible Yes
Absorbance, Fluorescence and Luminescence true
Plate type(s) 6 – 384 well plates

Absorbance photometric range

Wavelength range 200–1000 nm
Wavelength selection 1.0 nm increments
Wavelength bandwidth ≤ 5 nm full width half maximum
Wavelength accuracy ± 2 nm across wavelength range
Wavelength precision (repeatability) ± 0.2 nm
Photometric range 0–4.0 OD
Photometric resolution 0.001 OD
Photometric accuracy, linearity < ±0.006 OD ±1.0%, 0–3 OD
Stray light ≤ 0.05% at 230 nm
Photometric precision (repeatability) < ±0.003 OD ±1.0%, 0–3 OD

Fluorescence intensity performance

Wavelength range 250–850 nm
Dynamic range > 6 decades
Sensitivity (fluorescein) 1 pM

Luminescence performance

Wavelength range 300–650 nm
Dynamic range > 7 decades
Sensitivity (ATP) 2 pM
Cross-talk <0.1% (white 96-well half-area microplate)

General performance

Plate formats 6, 12, 24, 48, 96, 384-well plates ANSI/SLAS
Light source Two high-energy xenon flash lamps as the light source
Shaking Linear, orbital, and double orbital
Reading capabilities Microplates, cuvettes (via adapter)
Detectors Ultra-cooled Photomultiplier Tube and Silicon photodiode
Temperature control 4°C above ambient to 45°C
Temperature uniformity ±1°C at 37°C
Temperature accuracy ±2°C at 37°C
Spectral scanning Abs
Endpoint reading Abs, FI, Lum
Kinetic reading Abs, FI, Lum
Well scanning Over 20 by 20

Standard Filter Cubes

Dichroic 405, 508, 555
Filter Name FL-465, FL-535, FL-595
Fluorophore/assay Coumarin derivative (AMC), AF350, Fluorescein/FITC, Alamar blue
Excitation 360/35, 485/20, 535/25
Emission 465/35, 535/25, 595/35

Optional Filter Cubes

Filter Name FL-360, FL-565, FL-635, FL-680, FL-810
Fluorophore/assay Tryptophan, Nano orange/Quant IT protein assay, Alexa Fluor 594, Texas Red, Alexa Fluor 647/cy5, Alexa Fluor 750/cy7
Excitation 280/20, 465/35, 590/20, 625/35, 710/50
Dichroic 310, 508, 610, 655, 765
Emission 360/35, 565/30, 635/25, 680/20, 810/40

Industry-leading software Industry-leading software

Enjoy an extensive library of protocols, advanced reader control, and easy data management and analysis with our best-in-class Softmax Pro Software.

Expanded wavelength range Expanded wavelength range

Xenon flash lamps allow you to excite samples in the UV range where halogen does not.

Approachable design Approachable design

Effortless functionality provides users with everything they need to run their most crucial applications quickly and without complications.

AutoPMT technology AutoPMT technology

Our AutoPMT feature allows a wide range of concentrations to be prepared and read on one plate, avoiding saturation (or the loss of signal) and saving you time and money.

Absorbance

Cellular Signaling

Cellular signaling allows cells to respond to their environment as well as to communicate with other cells. Proteins located on the cell surface can receive signals from the surroundings and transmit information into the cell via a series of protein interactions and biochemical reactions that comprise a signaling pathway. Multicellular organisms rely upon an extensive array of signaling pathways to coordinate the proper growth, regulation, and functioning of cells and tissues. If signaling between or within cells is dysregulated, inappropriate cellular responses may lead to cancer and other diseases.

ELISA

ELISA (Enzyme-linked immunosorbent assays)

Enzyme-linked immunosorbent assays (ELISAs) are used to measure the amount of a specific protein, using a microplate format, and results are most often detected via absorbance in the visible wavelength range. Chemiluminescent and fluorescent ELISA formats offer enhanced sensitivity for accurate quantitation of less abundant analytes.

Fluorescence

Many fluorescence-based assays include cell viability, GPCR activity, and fluorescent nucleic acid quantification.

Luminescence

Nucleic Acid (DNA/RNA) Quantitation and Analysis

Nucleic acids are large biomolecules common to all known life forms. Deoxyribonucleic acid (DNA) consists of a double strand of pairs of nucleotides, while ribonucleic acid (RNA) is typically a single strand. In DNA, the nucleotides are adenine, cytosine, guanine, and thymine, while RNA contains uracil instead of thymine. DNA makes up the genetic material of all organisms, encoding the information cells need to synthesize proteins.

Protein Detection, Quantitation and Analysis

Protein detection, quantitation, and analysis are central to the investigation of a wide variety of biological processes. Measuring the concentration of protein is necessary to processes ranging from protein purification and labeling to sample preparation for electrophoresis. Protein can be quantitated directly via absorbance at 280 nm, or indirectly using colorimetric (BCA, Bradford, etc.) or fluorometric methods offering advantages such as greater sensitivity. To identify and measure a specific protein within a complex sample, for example, serum or cell lysate, an ELISA may be used.