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FilterMax F3 & F5 – Multi-Mode Microplate Readers

Filter-based detection for absorbance, fluorescence, glow luminescence, and more

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Affordability and unmatched assay performance in filter-based microplate readers

The FilterMax™ F3 and F5 Multi-Mode Microplate Readers offer a wide set of features at an affordable price-to-performance ratio. They provide absorbance, fluorescence, glow luminescence, and other read modes for programmable endpoint, kinetic, multiple-wavelength linear scan and area scan measurements.

Get great performance with LEDs

The readers employ patented high-power light emitting diodes (LED) that are high powered at critical wavelengths, with an optimized light source for a specific dye or application.

Count single photons

The photomultiplier tube (PMT) detector operates in photon counting mode, affording increased sensitivity for red dyes with a linear dynamic range of over six orders of magnitude.

Get tailored data analysis

Save time from exporting data to external applications. SoftMax Pro Software provides more than 160 predefined yet powerful experiment protocols and programming capabilities for quick data analysis.

FilterMax F3

FilterMax F5
General specifications
Read modes Absorbance Fluorescence (top read) Luminescence (top read) Absorbance Fluorescence (top/bottom read)
Luminescence (top read)
Time-Resolved Fluorescence (top read) Fluorescence Polarization (top read)
Wavelength ranges Abs: 340 – 650 nm
FL Ex: 340 – 630 nm
FL Em: 390 – 650 nm
Lumi: 400 – 650 nm		 Abs: 230 – 650 nm
FL Ex: 230 – 650 nm
FL Em: 390 – 700 nm
Lumi: 400 – 700 nm
TRF: 340 – 700 nm
FP Ex: 300 – 650 nm
FP Em: 390 – 700 nm
Wavelength selection Filters Filters
Absorbance photometric accuracy/linearity <±0.75% and ±0.010 OD from 0.1 OD to 3.0 OD (>399 nm)
<±0.75% and ±0.010 OD from 0.1 OD to 2.5 OD (<400 nm)		 <±0.75% and ±0.010 OD from 0.1 OD to 3.0 OD (>399 nm)
<±0.75% and ±0.010 OD from 0.1 OD to 2.5 OD (<400 nm)
Absorbance photometric precision/repeatability <±0.5% and ±0.005 OD at 2.0 OD (400 nm to 650 nm)
<±0.5% and ±0.005 OD at 1.0 OD (230 nm to 399 nm)		 <±0.5% and ±0.005 OD at 2.0 OD (400 nm to 650 nm)
<±0.5% and ±0.005 OD at 1.0 OD (230 nm to 399 nm)
Sensitivity top read optimized FL: 3 fmol/well in 100 μL fluorescein 96 well (15 pM), 0.4 fmol/well in 75 μL fluorescein 384 well (5.3 pM)
Lumi: 2 fmol/well in 200 μL ATP 96 well (10 pM), 0.75 fmol/well in 50 μL ATP 384 well (15 pM)		 FL: 0.3 fmol/well in 200 μL fluorescein 96 well (1.5 pM) , 0.1 fmol/well in 75 μL fluorescein 384 well (1.3 pM)
Lumi: 2 fmol/well in 200 μL ATP 96 well (10 pM), 0.75 fmol/well in 50 μL ATP 384 well (15 pM)
TRF: 30 amol/well in 200 μL Europium 96 well (0.15 pM), 15 amol/well in 100 μL Europium 384 well (0.15 pM)FP: 1 mP at 10 nM fluorescein 96 well, 1.5 mP at 10 nM fluorescein 384 well
Light source(s) High power LEDs Deuterium lamp High power LEDs Deuterium lamp
Detector(s) Silicon photodiode Photomutiplier tube Silicon photodiode Photomutiplier tube
Read types (endpoint, kinetic, etc) Endpoint Kinetic Well scan Endpoint Kinetic Well scan
Read times Abs: 27 seconds
FL: 29 seconds
Lumi: 28 seconds		 Abs: 27 seconds
FL: 29 seconds
Lumi: 28 seconds
FP: 54 seconds
Plate shaking Linear Orbital Linear Orbital
Robotics / automation Có Có
Plate type(s) 6-384 well plates 6-1536 well plates
Temperature control Không Ambient + 4°C to 45°C
Dimensions H 24 cm x W 39 cm x D 58 cm H 24 cm x W 39 cm x D 58 cm
Validation tools Multi-mode validation plate SoftMax® Pro GxP Software Multi-mode validation plate SoftMax® Pro GxP Software

Visible absorbance

This reader provides visible absorbance, top-reading fluorescence intensity, and glow luminescence modes in 96- to 384-well plates with wavelength selection options from 340-650 nm.

UV and visible absorbance

This reader provides UV and visible absorbance, top and bottom fluorescence intensity, glow luminescence, FP, and TRF modes in 6- to 1536-well plates with wavelength selection options from 230-750 nm.

Absorbance

Common absorbance applications and assays including ELISAs, nucleic acid and protein quantitation, and microbial growth.

Cell viability

Cell viability

Cell line development requires the discovery of single cell-derived clones that produce high and consistent levels of the target therapeutic protein. A critical first step in the process is the isolation of single, viable cells. Single cells proliferate to form colonies that can then be assessed for productivity of the target therapeutic protein. Viability and growth rates of single cell-derived clones can be characterized on the CloneSelect Imager and the SpectraMax i3x microplate reader and imaging cytometer.

ELISA

ELISA (Enzyme-linked immunosorbent assays)

Enzyme-linked immunosorbent assays (ELISAs) are used to measure the amount of a specific protein, using a microplate format, and results are most often detected via absorbance in the visible wavelength range. Chemiluminescent and fluorescent ELISA formats offer enhanced sensitivity for accurate quantitation of less abundant analytes.

Fluorescence

Many fluorescence-based assays include cell viability, GPCR activity, and fluorescent nucleic acid quantification.

Fluorescence Polarization (FP)

Fluorescence polarization (FP) is a technique that is widely used to monitor binding events in solution. It can be used to assess biomolecular interactions, including protein-antibody binding and DNA hybridization, as well as enzyme activity, and it has been adapted to basic research as well as high-throughput screening.

Fluorescent Protein Detection

Fluorescent proteins have become enormously popular as tools for monitoring biological events in vivo. In addition to green fluorescent protein (GFP) from the jellyfish Aequorea victoria, there are now numerous others available from other species of jellyfish and reef coral. These proteins can be expressed in a diverse range of cells and organisms, where they are used to track many cellular processes, including protein synthesis and translocation, gene induction, and cell lineage.

Luminescence

The key luminescence-based assays include dual-luciferase reporter gene, chemiluminescent ELISA, cytotoxicity, and BRET.

Mycoplasma Monitoring

Giám sát Mycoplasma

Mycoplasma, the smallest and simplest of the prokaryotes, are common contaminants of cell cultures. Symptoms of mycoplasma contamination include a reduction in the rate of proliferation and changes in cellular responses, including gene expression

TRF, TR-FRET và HTRF

Learn all about time-resolved fluorescence – TRF and TR-FRET including HTRF assays. We’ll provide applications resources to aid in your research, including kinase assays, cellular signaling pathways, protein-protein interactions, cell cytotoxicity, and more.

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