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NanoWizard 4 XP BioScience – Atomic Force Microscopes for Life Science

Extreme performance and ease of use for applications in Life Science and Soft Matter research ranging from single molecules to living cells and tissues.

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The NanoWizard 4 XP BioScience atomic force microscope combines atomic resolution and fast scanning with rates of up to 150 lines/sec and a large scan range of 100µm in one system. It is designed to provide highest mechanical and thermal stability on inverted optical microscopes during long term experiments on samples ranging from single molecules to living cells and tissues.

Performance (150 lines/sec and 100 µm scan range)

Understand the complexity of biological systems: Trigger events via precise control of temperature, pH, and fluid/gas exchange. Observe response to stimuli and sample dynamics.

High Resolution

Structural analysis and nanomechanical characterization of biological samples. Precise investigation of molecular mechanisms, biochemical interactions, and cell mechanics.

Flexibility

Simultaneous, correlated measurements with techniques like FRET, TIRF, FLIM, FCS, epifluorescence, confocal laser scanning and STED microscopy.

Standard Operating Modes

Imaging Modes

  • Now with PeakForce Tapping
  • Contact Mode with Lateral Force Microscopy (LFM)
  • Tapping Mode™ with PhaseImaging™

Force Measurements

  • Static and dynamic spectroscopy
  • Advanced force mapping

Optional Modes

  • Fast Scanning option with up to 150 lines/sec
  • Fast QI Advanced mode for quantitative data, perfect for soft samples
  • Mechanical properties such as adhesion, elasticity, stiffness, deformation
  • Conductivity and charge distribution mapping
  • Contact Point Imaging (CPI) with zero force
  • Molecular recognition imaging for binding site mapping
  • Advanced AC modes such as FM and PM with Q-control & Active Gain Control
  • Higher harmonics imaging
  • Kelvin Probe Microscopy and SCM
  • MFM and EFM (see also QI mode)
  • Conductive AFM (see also QI mode)
  • STM (Scanning Tunneling Microscopy)
  • Electrical spectroscopy modes
  • Piezoresponse Microscopy for high voltages
  • Electrochemistry with temperature control and optical microscopy
  • NanoLithography
  • NanoManipulation
  • Nanoindentation
  • Scanning Thermal AFM
  • FluidFM® solution from Cytosurge
  • ExperimentPlanner for designing a specific measurement workflow
  • RampDesigner™ for custom clamp and ramp experiments
  • ExperimentControl feature for remote experiment control
  • DirectOverlay 2 for combined AFM and optical microscopy
  • Additional XY or Z sample movement stages available with CellHesion®, TAO™ and HybridStage™ modules

High-resolution imaging with extreme performance

The NanoWizard 4 XP BioScience comes with:

  • PeakForce Tapping® for easy imaging
  • Fast Scanning option with up to 150 lines/sec
  • NestedScanner Technology for high-speed imaging of surface structures up to 16.5µm with outstanding resolution and stability
  • New tiling functionality for automated mapping of large sample areas
  • V7 Software with revolutionary new workflow-based user interface
  • DirectOverlay™ 2 software for perfect integration and data correlation with advanced fluorescence microscopy platforms
  • Vortis™ 2 controller for high-speed signal processing and lowest noise levels

Automated mapping of large sample areas with new tiling functionality

The HybridStage™ or Motorized Precision Stage transforms experiments by enabling direct access to a large sample area, with automated, motorized movement to selected positions, grids and mapping regions.

Begin with the DirectOverlay 2 optical calibration, and then select a region for optical tiling up to millimeters in size.

Precise motor movements automatically bring the whole sample into view, making it easy to select regions and features for further investigation. A single click navigates from point to point or MultiScan experiments automate a sequence of measurements at selected points.

Perfect optical integration provides true correlative microscopy

The NanoWizard 4 XP, with its unique tip-scanning technology and fast imaging capabilities, is ideal for taking advantage of the synergy between AFM and super-resolution microscopy.

The NanoWizard 4 XP is compatible with a wide range of platforms, such as those from Zeiss (PALM/STORM, SIM), Leica (STED), PicoQuant (STED), Nikon (SIM, STORM) and Abberior (STED).

The 980nm laser option for the AFM head allows the simultaneous use of optical microscopes and focus stabilization systems, critical for long term experiments, and avoids conflicts with fluorescence or spectroscopy measurements.

Outstanding quantitative data from molecules, cells and tissues

QI™ Advanced, based on real force curves, offers both astounding speed and resolution for applications ranging from single molecules to living cells. The quantitative data allows precise and fast analysis of mechanical or biochemical interactions, e.g., localization of binding sites, directly overlaid with fluorescent labelling and topography with Molecular Recognition Imaging. Advanced batch processing options include multiple models for modulus fitting and can reveal surface topography at zero force with Contact Point Imaging (CPI).

  • Fast Scanning option with up to 150 lines/sec for tracking dynamic processes
  • Now with Bruker’s exclusive PeakForce Tapping as standard
  • Large scan field of 100 × 100 × 15 µm3 and atomic lattice resolution on inverted microscopes
  • New workflow-based user interface for ergonomics and ease of operation
  • New tiling functionality for automated mapping of large sample areas together with the HybridStage
  • Enhanced DirectOverlay 2 mode for most precise correlative microscopy
  • New Vortis 2 controller with high-speed low-noise DACs and cutting-edge position sensor readout technology
  • Highest flexibility and upgradeability with a broad range of modes and accessories

Bruker’s BioAFMs allow life science and biophysics researchers to further their investigations in the fields of cell mechanics and adhesion, mechanobiology, cell-cell and cell-surface interactions, cell dynamics, and cell morphology.

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