Online Support

Hotline

0962 28 23 24

Ficoll™ PM 70 – Density gradient media

Highly stable sucrose polymer with an average molecular weight 70 000, suitable for use as perfusion media in laboratory studies on dextran-sensitive animals.

Category: Tags: , , Brand:

Highly stable sucrose polymer with an average molecular weight 70 000, suitable for use as perfusion media in laboratory studies on dextran-sensitive animals.
  • Displays minimal toxicity
  • Has favorable viscosity and osmotic properties
  • Supplied as spray-dried powders.

Highly soluble

High molecular weight sucrose-polymers formed by copolymerization of sucrose with epichlorohydrin. The molecules are highly branched and the high content of hydroxyl groups leads to very good solubility in aqueous media.

Application Specifically intended for use in perfusion media for laboratory studies on dextran-sensitive animals.
Endotoxin Activity Max. 0.12 EU/mg
Estimated shelf life from manufacture date 5 years
Storage conditions 4–30°C
Stability Stable in alkaline and neutral solutions, but are rapidly hydrolyzed in solution at pH 3, especially at elevated temperature
Physical State Solid
Physical Form Spray-dried powder
Molecular Weight (Mr) Max. ~ 8 × 104
Molecular Weight (Mr) Min. ~ 6 × 104
Reactivity and Stability The reactivity and stability of Ficoll PM70 are determined by its hydroxyl groups and the glycosidic bonds in the sucrose residues. It is stable in alkaline and neutral solutions. At pH values lower than 3, it is rapidly hydrolyzed, especially at elevated temperatures. In neutral solutions, however, Ficoll PM 400 can be sterilized by autoclaving at 110°C for 30 min without degradation. Strong oxidizing and reducing agents should be avoided.
Stokes Radius ~ 5.1 nm
Color White
Specific Rotation (alpha)20 50°-65°
Intrinsic Viscosity ~ 10 ml/g
Solubility It is delivered as a spray-dried powder and is therefore readily soluble in aqueous media when it is added slowly with concomitant stirring. Any lumps formed during this procedure will dissolve fairly quickly. Concentrations up to 50% (w/v) can easily be attained
Certificate of Analysis Yes
Application Specifically intended for use in perfusion media for laboratory studies on dextran-sensitive animals.
Endotoxin Activity Max. 0.12 EU/mg
Estimated shelf life from manufacture date 5 years
Storage conditions 4–30°C
Stability Stable in alkaline and neutral solutions, but are rapidly hydrolyzed in solution at pH 3, especially at elevated temperature
Physical State Solid
Physical Form Spray-dried powder
Molecular Weight (Mr) Max. ~ 8 × 104
Molecular Weight (Mr) Min. ~ 6 × 104
Reactivity and Stability The reactivity and stability of Ficoll PM70 are determined by its hydroxyl groups and the glycosidic bonds in the sucrose residues. It is stable in alkaline and neutral solutions. At pH values lower than 3, it is rapidly hydrolyzed, especially at elevated temperatures. In neutral solutions, however, Ficoll PM 400 can be sterilized by autoclaving at 110°C for 30 min without degradation. Strong oxidizing and reducing agents should be avoided.
Stokes Radius ~ 5.1 nm
Color White
Specific Rotation (alpha)20 50°-65°
Intrinsic Viscosity ~ 10 ml/g
Solubility It is delivered as a spray-dried powder and is therefore readily soluble in aqueous media when it is added slowly with concomitant stirring. Any lumps formed during this procedure will dissolve fairly quickly. Concentrations up to 50% (w/v) can easily be attained
Application Specifically intended for use in perfusion media for laboratory studies on dextran-sensitive animals.
Endotoxin Activity Max. 0.12 EU/mg
Estimated shelf life from manufacture date 5 years
Storage conditions 4–30°C
Stability Stable in alkaline and neutral solutions, but are rapidly hydrolyzed in solution at pH 3, especially at elevated temperature
Physical State Solid
Physical Form Spray-dried powder
Molecular Weight (Mr) Max. ~ 8 × 104
Molecular Weight (Mr) Min. ~ 6 × 104
Reactivity and Stability The reactivity and stability of Ficoll PM70 are determined by its hydroxyl groups and the glycosidic bonds in the sucrose residues. It is stable in alkaline and neutral solutions. At pH values lower than 3, it is rapidly hydrolyzed, especially at elevated temperatures. In neutral solutions, however, Ficoll PM 400 can be sterilized by autoclaving at 110°C for 30 min without degradation. Strong oxidizing and reducing agents should be avoided.
Stokes Radius ~ 5.1 nm
Color White
Specific Rotation (alpha)20 50°-65°
Intrinsic Viscosity ~ 10 ml/g
Solubility It is delivered as a spray-dried powder and is therefore readily soluble in aqueous media when it is added slowly with concomitant stirring. Any lumps formed during this procedure will dissolve fairly quickly. Concentrations up to 50% (w/v) can easily be attained

Related products

GenVoy-ILM™ - T cell kits for mRNA

Cell Therapy Systems

GenVoy-ILM™ – T cell kits for mRNA

Sepax C-Pro - Cell Processing Instrument

Automated Cell Processing System

Sepax C-Pro – Cell Processing Instrument

ÄKTA readyflux™ - Tangential Flow Filtration Systems

Bioprocess Filtration

ÄKTA readyflux™ – Tangential Flow Filtration Systems

GenVoy-ILM™ - Reagents and Kits

Cell Therapy Systems

GenVoy-ILM™ – Reagents and Kits

Xcellerex™ - X-platform bioreactors

Microbioreactor

Xcellerex™ – X-platform bioreactors

NanoAssemblr™ Ignite - Heating Packages

Cell Therapy Systems

NanoAssemblr™ Ignite – Heating Packages

NanoAssemblr™ Ignite and Ignite+ cartridges

Cell Therapy Systems

NanoAssemblr™ Ignite and Ignite+ cartridges

ÄKTA ready™ - Chromatography Systems

Chromatography

ÄKTA ready™ – Chromatography Systems