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Ficoll-Paque™ PREMIUM – Density gradient media

Ready-to-use sterile density gradient media designed for the preparation of mononuclear cells from peripheral blood, bone marrow and cord blood.

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Ready-to-use sterile density gradient media designed for the preparation of mononuclear cells from peripheral blood, bone marrow and cord blood.

  • Manufactured under a Quality Management System certified to ISO 13485.
  • Low levels of endotoxin (< 0.12 EU/mL) secured and tested.
  • Cytotoxicity grade 0.
  • Choice of densities: 1.077, 1.084, and 1.073 g/mL.
  • A Regulatory Support File (RSF) is available for Ficoll-Paque PREMIUM products.

Ficoll-Paque PREMIUM vs Ficoll-Paque PLUS

All Ficoll-Paque PREMIUM products differ from Ficoll-Paque PLUS in that they are manufactured under a Quality Management System certified to ISO 13485.

How it’s used

  • Ficoll-Paque PREMIUM: Optimized for the isolation of mononuclear cells from human peripheral blood, bone marrow, and umbilical cord blood.
  • Ficoll-Paque PREMIUM 1.084: Can be used for isolating a broad range of human mononuclear cells including those of a higher density and for separating blood cells from mice or rats.
  • Ficoll-Paque PREMIUM 1.073: Can be used when isolating lower-density human mononuclear cells (e.g. mesenchymal stromal cells or monocytes).
Application Optimized for the isolation of mononuclear cells from human peripheral blood, bone marrow, and umbilical cord blood.
Endotoxin Activity Max. < 0.12 EU/ml
Properties Ficoll-Paque PREMIUM products constitute a range of sterile, ready-to-use density media containing Ficoll PM400, sodium diatrizoate and disodium calcium EDTA in water for injection (WFI). The densities and osmolalities of the different products have been optimized for the isolation of mononuclear cells from blood and bone marrow
Estimated shelf life from manufacture date 3 years
Storage conditions 4–30°C
Stability Ficoll-Paque PREMIUM products are provided as sterile solutions which are stable for at least 3 yr when stored between 4°C and 30°C and protected from light. Deterioration of Ficoll-Paque PREMIUM products is indicated by the appearance of a yellow color or particulate material in the solution
Physical State Liquid
Osmolality 288-310 mOsm/kg
pH Range 5.5–7.5
Color Colorless to slightly yellow
Certificate of Analysis Yes
Application Optimized for the isolation of mononuclear cells from human peripheral blood, bone marrow, and umbilical cord blood.
Endotoxin Activity Max. < 0.12 EU/ml
Properties Ficoll-Paque PREMIUM products constitute a range of sterile, ready-to-use density media containing Ficoll PM400, sodium diatrizoate and disodium calcium EDTA in water for injection (WFI). The densities and osmolalities of the different products have been optimized for the isolation of mononuclear cells from blood and bone marrow
Estimated shelf life from manufacture date 3 years
Storage conditions 4–30°C
Stability Ficoll-Paque PREMIUM products are provided as sterile solutions which are stable for at least 3 yr when stored between 4°C and 30°C and protected from light. Deterioration of Ficoll-Paque PREMIUM products is indicated by the appearance of a yellow color or particulate material in the solution
Physical State Liquid
Osmolality 288-310 mOsm/kg
pH Range 5.5–7.5
Color Colorless to slightly yellow
Certificate of Analysis Yes

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