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DM4 B & DM6 B – Upright Microscopes

Crisp Images with Differential Interference Contrast (DIC)

For unstained biological specimens, especially thick samples such as brain slices, observing fine details can be challenging due to low contrast – many structures appear only as faint gray shapes, making it difficult to distinguish individual cells.

The Leica DM4 B and DM6 B microscopes support Differential Interference Contrast (DIC) – an ideal contrast method that delivers images with exceptional depth, sharpness, and contrast without the need for staining.

DIC works by exploiting differences in the refractive index of various regions within the sample. As light passes through these areas, it creates images with a 3D-like relief effect, allowing clear visualization of fine cellular structures. This makes DIC a top choice for histological studies, neuroscience, and live-cell imaging applications.

Automated Upright Microscope System for Life Science and Clinical Applications Leica DM4 B Fully Automated Upright Microscope System for Life Science Research Leica DM6 B
Display Shows all microscope settings at a glance Controls the microscope conveniently and intuitively
Focus Mechanical Mechanical & Motorized
Stage Mechanical (motorized optional) Motorized (optional)
LAS X Navigator Software Yes, with mot. or scanning stage Yes
Transmitted Light Illumination LED LED
Transmitted Light Contrast Methods BF, PH, DF, POL, DIC BF, PH, DF, POL, DIC
Fluorescence Axis Fully automated Fully automated, with Internal Filter Wheel (IFW) and Excitation Manager (optional)
Fluorescence Illumination LED or EL6000 (metal halide) LED or EL6000 (metal halide)

See More in Less Time

Saving time in image acquisition means you have more time for what is really at the heart of your science. Here are just a few ways to speed up your workflow.

  • See up to 10.000x more of your sample with the LAS X Navigator. Set up high resolution image acquisition for slides, dishes and multiwell plates
  • Take full advantage of your sCMOS camera, for example the Leica DFC9000! The 19-mm field of view camera port perfectly fits the dimensions of common sCMOS sensors.
  • Make your slide examination faster at the highest resolution.
  • Choose the objective that best fits your application – from a range of more than 300 outstanding optics. Just one example: the unique 1.25x overview objective for a perfect wideview.

Free Your Mind!

Focus on the result you would like to achieve rather than the process of getting to it. Intelligent Automation does the work for you: It solves multi-step routines with the press of a button, makes your work life easier, saves time, and allows you to focus on your experiment, not on complex microscope settings.

  • Change contrast with the push of a button – everything you need automatically slides into place.
  • Acquire publication-ready images.
  • Use Light and Contrast Management to simplify your work – for example the Fluorescence Intensity Manager (FIM), fast Internal Filter wheel (IFW) and Excitation Manager (ExMan) for fluorescence images, or Koehler light management for perfect Koehler images.

Exactly What You Need

You can configure the microscope that fits your need and budget with the Leica DM4 B and Leica DM6 B. Optimize your system to your application with different contrast methods and automated functions.

LED illumination lights your sample homogenously and with a constant color temperature, while also saving energy and avoiding bulb changes due to the LED’s lifetime of up to 25,000 hours

Make Your Microscope an Imaging System

Leica Microsystems provides powerful cameras and workflow-based software to complete your integrated imaging system.

  • Leica Application Suite X (LAS X) is our easy-to-use workflow-based software which guides you step by step to acquire the images you need.
  • Leica microscope cameras cover a broad range of applications from brightfield to fluorescence applications – or even one camera for multi-purposes both such as our Leica DFC7000 T camera