The CloneSelect Imager FL is a next-generation fluorescent imaging solution for the assurance of monoclonality and automated confluence across diverse cell types
Demonstrating that cell lines are monoclonal – or that a gene was edited as expected – can be a time-consuming and highly-subjective process when relying on conventional technologies. The CloneSelect® Imager and CloneSelect® Imager FL are a high-throughput automated solutions for imaging and analyzing mammalian cells. Tracking the formation of a colony from a single cell is effortless as barcoded plates are tracked over time. Automated acquisition and analysis provides accurate, objective, and consistent results.
With high-resolution white light imaging, the CloneSelect Imager provides automated confluence, monoclonality assurance and industry leading acquisition times with the ability to image a 96-well plate in under two minutes.
The all new CloneSelect Imager FL features high contrast multichannel fluorescent and white light imaging that allows for accurate single cell detection and proof of monoclonality at day 0. Streamline your workflow with comparative confluence assays to identify and verify gene edits.
Demonstrated IND success
The Monoclonality Report feature streamlines the creation of supporting documentation for regulatory agencies. Reports are automatically generated based on parameters you select. The Monoclonality Report is an audit-ready document that supports filing for an Investigational New Drug (IND) Application with the FDA. (21 CFR Part 312)
Multichannel imaging and automated confluence
Algorithms are optimized for accurate cell detection and address varying cell types and conditions. Publication-ready high-resolution imaging provides automatic confluence analysis and monoclonality assurance.
Rapid single cell confirmation
The imager delivers industry leading acquisition times, imaging a 96-well plate in as little as two minutes and can verify monoclonality from day zero with fluorescence.
White light and fluorescent imaging
Image every well in every plate with rapid acquisition times with label-free white light. Multichannel fluorescence imaging provides additional confidence of monoclonality and comparative confluence assays (red vs green).
Image a 96-well plate in under two minutes. Compatible with adherent or settled suspension cell types such as CHO, HEK, hybridomas, iPSCs, and many other cell types.
High-resolution images
High-speed fluorescence and high-resolution white light imaging with accurate detection of single cells including debris. Image visualization and data tracking over multiple days.
The software automatically calculates confluence measurements and generates growth curves, heatmaps, and image montages. Measurements for every well are automatically tracked over time. Streamline multiple steps (imaging, sample tracking, data analysis and report generation) in the cell line development workflow for IND filing.
Intelligent analysis with easy-to-use software
The software automatically calculates confluence for each imaging time point. Growth curve, image montage, total growth, and mean rate are generated automatically and are exportable. Guided software user interface allows for simple 1-hour training.
Intelligent analysis with easy-to-use software
The software automatically calculates confluence for each imaging time point. Growth curve, image montage, total growth, and mean rate are generated automatically and are exportable. Guided software user interface allows for simple 1-hour training.
(1) Image
Seed one cell per well and image at any point
- Optimize clonal outgrowth – The system is particularly useful for optimizing clonal outgrowth strategies when platform approaches are not suitable e.g. when investigating new cell lines or variants
- Diverse cell types – Compatible with adherent or settled suspension cell types such as CHO, HEK, hybridomas, IPSCs, and many other cell types.
(2) Analyze
Assess cell growth objectively and quantitatively
Track and view growth of every cell line
Growth curves calculated and displayed
Electronically track and store plate data: cell confluence, cell number estimation, and growth curve
(3) Report
Verify monoclonality
After initial seeding, CloneSelect Imager system can image every well, at any time point, using a ‘loci of growth’ functionality to highlight those wells that contain a single colony.
- Focus on wells with a single loci of growth and view image history to verify monoclonality with the CloneSelect Imager using white light
- With the CloneSelect Imager FL automatically verify monoclonality from day zero with fluorescence
Monoclonality Report provides image evidence of monoclonal cell bodies
With a few simple clicks, the Monoclonality Report feature on the CloneSelect™ Imager (CSI) objectively organizes the supporting image evidence needed to establish clonality into an easily shareable report, saving researchers hours typically required to do the same process manually.
- Easily identify and select single-cell and artifact regions to include in report
- Export high-resolution images of single cells, artifacts, and entire well (optional)
- Automatically identify single cells with fluorescence version of CloneSelect Imager
- Publish monoclonality report in PDF or Word Format
View entire well at final time point
Quickly determine clonality of a cell line by visually inspecting the presence of multiple colonies in a single well. For example, the well on the left shows one colony while the cell on the right shows two.
Monitor cell line development over time
To characterize the growth from a single cell to a colony, cell regions can be designated and adjusted for each time point in a series. The image bellow shows a single cell on Day 0 and two cells on Day 1 confirm monoclonality.
A single cell on Day 0 and two cells on Day 1 confirm monoclonality.
Divide an entire well into individual images
Export an entire well into 81 separate images to objectively confirm the absence of another cell. Below, a selected region is displayed over time.
Highlight regions to review non-cell objects
Selectively highlight parts of a well to differentiate cells from ambiguous objects. A selected artifact region and its corresponding images over time is shown here.
Cell Line Development
Cell line development is a critical step in the process of generating biopharmaceutical molecules, such as monoclonal antibodies. The process often begins with transfecting the host cell type with the DNA encoding the therapeutic protein of interest allowing for random or directed integration of target DNA into the host cell genome. Thousands of clones are screened to isolate the rare high producing cells, a manual and time-consuming process.
Cell viability
Cell line development requires the discovery of single cell-derived clones that produce high and consistent levels of the target therapeutic protein. A critical first step in the process is the isolation of single, viable cells. Single cells proliferate to form colonies that can then be assessed for productivity of the target therapeutic protein. Viability and growth rates of single cell-derived clones can be characterized on the CloneSelect Imager and the SpectraMax i3x microplate reader and imaging cytometer.
Drug Discovery & Development
For every drug that makes it to the finish line, another nine don’t succeed. This alarming failure rate can be traced to reliance on 2D cell cultures that don’t closely mimic complex human biology, often leading to inaccurate predictions of a drug’s potential and extended drug development timelines.
Label-Free Cell Migration
Wound healing assays are used in a range of disciplines to study cell migration, the coordinated movement of a cell population. A manual or standardized wound is generated on a monolayer of cells grown in microplates. The microplates can be screened against a library to study the effect on cell migration. The effect of a library of compounds or genes can be studied using this approach. The CloneSelect Imager enables the rapid imaging (<90 s per plate) and objective, automated analysis of cell migration.
Monoclonal Antibodies (mAbs)
Monoclonal antibodies (mAbs) originate from one unique parent cell, thus binding only to a single epitope. Monoclonal antibody discovery typically refers to the screening and identification of specific antibodies that target a specific epitope for the diagnosis and treatment of diseases, like the coronavirus for COVID-19.
Monoclonality
Cell line development and assurance of monoclonality are critical steps in the process of generating biopharmaceutical molecules, such as monoclonal antibodies. A cell line can be established following the isolation of a single viable cell robustly expressing the protein of interest. A key milestone in this process is documenting evidence of clonality. Documentation of clonality is typically image-based, whereby an image of a single cell is produced and included in regulatory filings.
Plasmid engineering in synthetic biology
Plasmids are small, circular DNA molecules that operate independently from chromosomal DNA. Central to molecular biology, they enable the study, manipulation, and production of genes, playing a vital role in cloning, gene therapies, and vaccine development.
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