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CellWizard Stage – Atomic Force Microscopes for Life Science

Automated, AI-guided, multi-chamber, in vitro imaging and nanomechanical analysis

Bruker’s CellWizard™ Stage delivers pioneering, multi-compartment AFM imaging and nanomechanical analysis capabilities for life science applications, greatly extending the range, speed, and versatility of experiments possible, and pushing the boundaries of performance on the nanoscale. It facilitates automated large-scale mapping, high resolution imaging, and nanomechanical analysis in each chamber, and is ideal for the in vitro investigation of challenging biological samples.

Multi-Chamber – Optimized productivity and higher throughput

Automated large-scale mapping, high resolution imaging, and nanomechanical analysis in each compartment

Enhanced In Vitro research capabilities

Advanced multiparametric analysis of molecules, bacteria, cells, tissues, and organoids in a multi-chamber environment, for statistically relevant datasets

AI-Assistance

Advanced machine-learning capabilities, automated identification and measurement of features of interest, and systematic, reproducible measurements

Redefining the Future of BioAFM

The CellWizard Stage embodies state-of-the-art precision engineering. Using artificial intelligence (AI), it identifies sample features of interest and navigates autonomously from well to well, performing systematic, reproducible, and highly accurate measurements in each compartment. This next-generation AFM accessory delivers automated, multiscale, and multiparametric analyses. The ability to investigate biological samples in vitro, over a large scan range, and in a multi-compartment environment, allows a more comprehensive analysis and a deeper understanding of complex biological mechanisms, such as cellular communication and immune response.

The CellWizard Stage is compatible with the Bruker BioAFM atomic force microscope platform and can be seamlessly integrated into advanced optical microscopes.

Superior Performance

  • Compatibility with select choice of commercially available multiwell plates and multi-compartment glass slides
  • Fast and precise positioning with state-of-the-art encoders and custom developed firmware
  • Operation with renowned Bruker modes and features, including PeakForce Tapping and SmartMapping
  • Innovative software packages, such as, the AI optical image-guided AutomaticNavigation feature and the extended DirectTiling feature that now includes optical stitching and background correction

New Possibilities

  • Trigger different enzymatic reactions in each well
  • Study cellular response to varying stimuli
  • Deliver separate active ingredients to biological targets
  • Study biofilm formation inhibition factors
  • Quantify adhesion and disaggregation on varying substrates

Unmatched Functionality

Outstanding Automation

CellWizard Stage encompasses state-of-the-art automation features and advanced machine-learning capabilities that significantly improve the accuracy and effectivity of repetitive workflows by alleviating user error and optimizing productivity.

The AI-guided optical segmentation tool uses optical images to identify sample features of interest and automatically creates MultiScan lists within or surrounding them.

Optical snapshots or scheduled rinsing steps can be added to the planned list, and the instrument will then run measurements autonomously, with the CellWizard Stage safely changing between compartments as specified.

The AI image segmentation can use either the pre-trained models provided or user-trained custom models tailored to individual applications.

Comprehensive Capabilities

Explore a wide spectrum of easy-to-use features and options, such as:

  • Extensive optical integration options that include the DirectOverlay 2 software feature and accessories for operation on inverted optical microscopes
  • Multi-chamber substrate supports:
    4-well plate holder, a holder for four separate 35mm Petri dishes, and a holder for two standard glass slides
  • Bruker BioAFM PetriDishHeater options for 35mm and 50mm dishes
  • SmartMapping option with free-hand ROI-selection and intelligent piezo and motor motion – ideal for challenging biological samples
  • PeakForce QNM, QI Advanced mode, and microrheology options for quantitative nanomechanical analysis
  • Hinge-clip cantilever holder for easy operation in confined chambers

Extensive Flexibility

CellWizard Stage can be seamlessly combined with advanced optical techniques, such as confocal and super-resolution microscopy, for enhanced analysis capabilities and precise, correlated, multiparametric measurements.

It is compatible with the Bruker BioAFM family of instruments and its extensive range of modes, add-ons, and accessories, e.g.:

  • Humidity control
  • QR code reader for probes
  • Nanolithography and Nanomanipulation
  • ExperimentPlanner
  • Cytosurge FluidFM® options
  • Focus tracking support

The CellWizard Stage delivers the flexibility necessary for interdisciplinary research facilities and collaborative scientific environments.